الوصف
Philadelphia chromosome is a reciprocal translocation of the long arms of chromosomes 9 and 22t (9;22). This translocation transposes the c-abl oncogene from chromosome 9q34 to the B cell receptor (BCR) gene on chromosome 22q11.
دواعي الإستعمال
Philadelphia chromosome is found in >90% of patients with chronic myelogenous leukaemia (CML) and 15-25% of patients with acute lymphoblastic leukaemia (ALL). The fused BCR/abl gene and its gene products provide specific markers for diagnosis and disease monitoring. Fluorescent in situ hybridisation (FISH) techniques rely on chromosome-specific and gene-specific DNA probes to identify numeric and structural chromosomal abnormalities. The detection of BCR/abl translocation by FISH is rapid; however, the sensitivity of this approach may be limited as, in about 5% of normal lymphocytes, artifactual colocalisation may be observed.
نوع العينة والكمية والشروط
3 ml EDTA Whole Blood (PCR) 5 ml Heparinised Whole Blood (FISH) Room Temperature
إحتياطات خاصة
Full patient history required. Collect in EDTA vacutainer tube. Avoid contamination with DNA of phlebotomist and send tube unopened. Avoid haemolysed samples. Do not freeze samples.
المعدل الطبيعي
As per Report