زراعه الدم بالطريقه الاوتوماتيكيه

Used in the isolation and identification of potentially pathogenic organisms causing bacteraemia, and to establish the diagnosis of endocarditis. Also important for diagnosis in cases of suspected sepsis, meningitis, osteomyelitis, arthritis, or acute untreated bacterial pneumonia. For other sources of bacteraemia, there may be a more intermittent shedding of organisms into the blood. It is recommended to collect at least two to three sets of blood cultures per septic episode, ideally from separate venipuncture sites, spaced about 30-60 minutes apart to increase detection sensitivity and to assist in defining the significance of possible contamination, chiefly coagulase negative staphylococci. Prior antimicrobial therapy may decrease yield.

Patient Preparation: The major difficulty in interpretation of blood cultures is potential contamination by skin flora. This difficulty can be markedly reduced by careful attention to the details of skin preparation and antisepsis prior to collection of the specimen.

10 ml Whole Blood/100 ml media. Room Temperature For infants: 1-5 ml whole blood/100 ml media.

Follow stringent aseptic technique. Special culture bottles, designed for aerobic and anaerobic organisms should be used. If bacterial endocarditis is present, all blood cultures may be positive due to continuous bacteremia.

No Growth after 5 days of incubation