Human Oncogenes, Colorectal Cancer Gene (K-Ras) Mutations

The K-ras gene is one of a family of three human ras genes (K-ras, H-ras, and N-ras). These encode small GTP binding proteins localised to the inner surface of the plasma membrane and are involved in transducing signals from receptor tyrosine kinases such as epidermal growth factor receptor. The ras proteins are activated on binding GTP and deactivated by intrinsic GTPase activity from two GTPase activating proteins (GAP). The ras oncogenes are activated by point mutations that prevent the activation of GTPase. The point mutations that trigger the oncogenic potential of ras have been detected in more than 80% of cases on codons 12 and 13.

K-ras mutations are one of the most common genetic lesions in human cancer. More than 50% of colorectal cancers display specific mutations in the K-ras gene, with an increasing frequency in larger and more advanced lesions. Multivariate analysis suggests that the presence of a mutation increases risk of recurrence and death.

3 ml EDTA Whloe Blood Room Temperature

Collect in EDTA vacutainer tube.Avoid contamination with DNA of phlebotomist and end tube unopened. Avoid haemolysed samples. Do not freeze samples.

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