Treponema pallidum Hemagglutination (TPHA), Serum


Syphilis, caused by the spirochete Treponema pallidum, is a chronic infection with many diverse clinical manifestations that occur in distinct stages. Tests for syphilis fall into four categories: (1) Direct microscopic examination, used when lesions are present; (2) Non-treponemal tests, used for screening. (3) Treponemal tests that are confirmatory. (4) Direct antigen detection tests currently used in research settings.


TPHA is a treponemal confirmatory test that detects the presence of Treponema pallidum specific antibodies in serum. The test is used in the diagnosis of syphilis. A positive result indicates past or current infection with Treponema pallidum.

Sample Type, Quantity & Conditions

1 ml Serum Stability: 3 Days at 2-8 °C

Special Precautions

Normal Range

Negative: < 1/80 Titre

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