Description
The ABO haptens are terminally located carbohydrate residues on the cell surface and in secretions that are biosynthesised by blood groupspecific glycosyltransferases encoded by the ABO locus on the long arm of chromosome 9. In total, 8 A (including cis-AB) and 6 B (including 2B(A)) subgroup alleles defined by missense mutations in exon 7 have been described.
Indications
ABO genotyping is a valuable complement to serology for correct determination of donor and patient ABO blood group status. As an independent tool in the clinical laboratory it can confirm a weakly expressed A or B antigen in inherited subgroups and also exclude B allele markers in the acquired B antigen phenotype. Although monoclonal antibodies recognising and agglutinating most weak subgroups exist, the risk for mistyping remains when using routine reagents.
Sample Type, Quantity & Conditions
3-5 ml EDTA Whole Blood Room Temperature
Special Precautions
Collect in EDTA vacutainer tube. Avoid contamination with DNA of phlebotomist. Avoid haemolysed samples. Do not freeze samples. Send tube unopened.
Normal Range
As per Report